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Image Search Results
Journal: Advanced Science
Article Title: Autocrine GDF10 Inhibits Hepatic Stellate Cell Activation via BMPR2/ALK3 Receptor to Prevent Liver Fibrosis
doi: 10.1002/advs.202500616
Figure Lengend Snippet: Therapeutic administration of GDF10‐Fc ameliorates hepatic fibrosis. A) Schematic drawing of the experimental procedure. B) Representative images of H&E and Sirius Red staining of liver from mice treated as in (A), scale bars, 50 µm ( n = 3). C,D) Measurement of the total liver collagen content by hydroxyproline assay (C) and serum ALT and AST levels (D) in the mice treated as in (A) ( n = 6). E, F) qPCR (E) ( n = 6) and Western blot (F) analysis of indicated genes in the liver of mice treated as in (A). G) Schematic drawing of the experimental procedure and representative images of H&E and Sirius Red staining of liver from AMLN diet‐induced fibrosis, scale bars, 50 µm ( n = 3). H,I) Measurement of the total liver collagen content by hydroxyproline assay (H) and serum ALT and AST levels (I) of the mice treated as in (G) ( n = 6). J,K) qPCR (J) ( n = 6) and Western blot (K) analysis of indicated genes in the liver of mice treated as in (G). L) Schematic drawing of the experimental procedure and representative images of H&E and Sirius Red staining of liver from the MCD diet‐induced fibrosis, scale bars, 50 µm ( n = 3). M,N) Measurement of the total liver collagen content by hydroxyproline assay (M) and serum ALT and AST levels (N) of the mice treated as in (L) ( n = 6). O, P) Western blot (O) and qPCR (P) ( n = 6) analysis of indicated genes in the liver of mice treated as in (L). Q) The proposed model of GDF10 prevents liver fibrosis. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 by the two‐tailed Student's t ‐test (G–P), one‐way ANOVA (B,C,D,F), or two‐way ANOVA (E).
Article Snippet: For the metabolic‐induced liver fibrosis model, mice were fed with the AMLN diet (D09100301, Research Diets Inc.) for 28 weeks starting at 3 months of age, or the
Techniques: Staining, Hydroxyproline Assay, Western Blot, Two Tailed Test